Atcc hl-60

I cryopreserved HL-60 cells in FBS/ 10% DMSO but unfortunately cannot recover these cells properly. The cells have a good appearance at day 1 and 2. However, significantly lower cells counts are

hl-60/s4 atcc ® crl-3306 ™ frozen 1.0 mL For-Profit: $632.00 Non-Profit: $537.20 Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e. HL-60 cells(CCL-240; ATCC, Manassas, VA) were maintained at 37°C in Iscove modifiedDulbecco medium supplemented with 10% (vol/vol) FBS and 1% (vol/vol) penicillin-streptomycin in a humidified atmosphere of 95% air and 5% CO2 Differentiation of HL-60 cells into PMN-like cells was induced by incubating them in 1.25% DMSO for 6 days. The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals.

25.10.2020

The ATCC HCoV-OC43 strain (VR-759) was grown on the HRT-18 rectal tumor HL-60. Human. Bone marrow. Monocyte. 3.25. Low. WI-38.

May 25, 2017 · HL-60 cell culture and treatment. Cells of the HL-60 line obtained from the American Type Culture Collection (ATCC, European branch in UK) were cultured in RPMI-1640 medium supplemented with 10% FBS without antibiotics at 37 °C in a 5% CO 2 and 95% humidified atmosphere.

Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 2 PubMed citations. Cell culture Human acute promyelocyte leukemia HL-60 cells and acute myeloid leukemia KG1a cells were purchased from the American Type Culture Collection (ATCC)(Manassas, VA, USA) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), penicillin (100 U/mL, Gibco), and streptomycin (100 µg/mL, Gibco). ..

Human HL-60 cells (ATCC-No: CCL-240) were isolated from a patient with AML (acute myeloid leukemia) and express FLT3 wild type. A Hematoxylen-Eosin stained paraffin section of a subcutaneous HL-60 xenograft is shown on the right. As routine quality controls, the cells are regularly checked for Mycoplasma contamination and authenticity (via STR DNA Typing). Expression data using western …

HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours. ATCC HL-60, Promyelocytic Leukemia, Frozen Manufacturer: ATCC CCL240 HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e.

HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. To ATCC Valued Customers, HL-60/MX1 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. hl-60-luc2 atcc ® ccl-240-luc2 ™ frozen 1.0 mL For-Profit: $1,310.00 Non-Profit: $825.30 HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent.

A Hematoxylen-Eosin stained paraffin section of a subcutaneous HL-60 xenograft is shown on the right. As routine quality controls, the cells are regularly checked for Mycoplasma contamination and authenticity (via STR DNA Typing). Expression data using western … Article Snippet: Cell lines The mycoplasma-free AML U937 (ATCC CRL-1593.2; French-American-British/FAB phenotype M5, monoblast), NB4 (M3, promyelocyte) [ ], HL-60 (ATCC 240-CCL; M2, myeloblast) and THP-1 (ATCC TIB-202; M5, monoblast) cells were cultured in complete RPMI 1640 medium supplemented with 5% FCS in a 5% CO2 humidified atmosphere at Human Aml Cell Lines Hl60, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 2 PubMed citations. Cell culture Human acute promyelocyte leukemia HL-60 cells and acute myeloid leukemia KG1a cells were purchased from the American Type Culture Collection (ATCC)(Manassas, VA, USA) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), penicillin (100 U/mL, Gibco), and streptomycin (100 µg/mL, Gibco).

2. of . 3. profiling Clone 15 HL-60 98052918 Human … Clone 15 HL-60 Other Collection No.: ATCC CRL-1964 Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: Clone 15 HL-60 (ECACC 98052918) Keywords: Human caucasian promyelocytic leukaemia Cell Line Description: The clone 15 HL-60 has been derived from HL-60 cells (ECACC catalogue no. 98070106) that had been cultured at an elevated … 22/05/2018 Compound Viability screens Drug sensitivity AUC (Sanger GDSC1) Drug sensitivity AUC (Sanger GDSC2) Drug sensitivity IC50 (Sanger GDSC1) Drug sensitivity IC50 (Sanger GDSC2) Drug sensitivity AUC (CTD^2) Drug sensitivity replicate-level dose (CTD^2) Drug sensitivity replicate-level dose (Sanger GDSC1) Drug sensitivity replicate-level dose (Sanger GDSC2) This reference provides a recommended procedure to transfect plasmid DNA into HL-60 Human lymphoblasts (ATCC Cat. No. CCL-240) using Lipofectamine® LTX Reagent (Cat.

Human. Bone marrow. Monocyte. 3.25. Low. WI-38. Human.

(f – i) Enlarged images of cells during separation from Figure 2 e. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research. With a free account, you can easily search, download, and analyze hundreds of high-quality genomes.

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HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e.

of . 3. profiling Clone 15 HL-60 98052918 Human … Clone 15 HL-60 Other Collection No.: ATCC CRL-1964 Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: Clone 15 HL-60 (ECACC 98052918) Keywords: Human caucasian promyelocytic leukaemia Cell Line Description: The clone 15 HL-60 has been derived from HL-60 cells (ECACC catalogue no. 98070106) that had been cultured at an elevated … 22/05/2018 Compound Viability screens Drug sensitivity AUC (Sanger GDSC1) Drug sensitivity AUC (Sanger GDSC2) Drug sensitivity IC50 (Sanger GDSC1) Drug sensitivity IC50 (Sanger GDSC2) Drug sensitivity AUC (CTD^2) Drug sensitivity replicate-level dose (CTD^2) Drug sensitivity replicate-level dose (Sanger GDSC1) Drug sensitivity replicate-level dose (Sanger GDSC2) This reference provides a recommended procedure to transfect plasmid DNA into HL-60 Human lymphoblasts (ATCC Cat. No. CCL-240) using Lipofectamine® LTX Reagent (Cat.